RNAP II antagonizes mitotic chromatin folding and chromosome segregation by condensin

RNAP II antagonizes mitotic chromatin folding and chromosome segregation by condensin

Blog Article

Summary: Condensin shapes mitotic chromosomes by folding chromatin into loops, but whether it does so by DNA-loop extrusion remains speculative.Although loop-extruding cohesin is stalled by transcription, the impact of transcription on condensin, which is enriched at highly expressed genes in many species, remains unclear.Using degrons of Rpb1 or the torpedo nuclease Dhp1XRN2 here to either deplete or displace RNAPII on chromatin in fission yeast metaphase cells, we show that RNAPII does not load condensin on DNA.Instead, RNAPII retains condensin in cis and hinders its ability to fold mitotic chromatin and to support chromosome segregation, consistent with the stalling of a loop extruder.

Transcription termination by Dhp1 limits such a hindrance.Our results shed light on the integrated functioning of condensin, and we argue rosy teacup dogwood that a tight control of transcription underlies mitotic chromosome assembly by loop-extruding condensin.